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Immortalized Rat Mesencephalic (AF5) Cells – SV40T (T155g)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Brain |
| Organism |
Rat (R. norvegicus) |
| Donor History |
E-14 rat embryo ,Mesencephalic Tissue |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow IV (TM004) + 10% FBS + 20 ng/ml rhFGF2 (Z101455) + 500 µg/ml Antibiotic G418 (G271) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method |
Immortalized via transfection with pCMV/SVE/neo carrying the N-terminal fragment of SV40 large T (T155g) |
| Full Information |
https://www.abmgood.com/immortalized-rat-mesencephalic-af5-cells-sv40t-t155g.html |
Immortalized Rat Mesencephalic (AF5) Cells – SV40T (T155g) are derived from E-14 rat embryos. Immortalization was preformed with the truncated SV40 large T (T155g) lacking the p53 binding domain gene via transfection. AF5 cells express glial and neuronal markers, indicative of mature phenotypic properties. It is a reliable source for central nervous system-related studies. Western blot to confirm presence of SV40 and markers.
