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Imunoanalýza
Immortalized Rat Steroidogenic Granulosa Cells Stably Expressing LH/CG Receptor (GLHR-15)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Ovary |
| Organism |
Rat (R. norvegicus) |
| Donor History | |
| Growth Properties |
Adherent ,multipolar |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow IV (TM004) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method |
Serial passaging and calcium phosphate mediated cotransfection by SV40 DNA and Ha-ras oncogene |
| Full Information |
Lutropin/choriogonadotropin (LH/CG) receptor, a receptor uniquely expressed in ovarian granulosa and testicular sertoli cells, play an important role in the regulation of oogenesis, spermatogenesis and steroid hormone production. Unfortunately the LH/CG receptor expression is often lost within 1-2 days of in vitro culture of primary cells. The Immortalized Rat Steroidogenic Granulosa Cells stably expressing LH/CG Receptor is responsive to luteinizing hormone (LH) or choriogonadtropin (hCG) stimulation and produces progesterone and 20α-OH-P as a result at levels comparable to primary cells. This cell line is useful in studying molecular mechanisms associated with gonadotropin stimulated steroidogenesis. Together with the Immortalized Rat Steroidogenic Granulosa Cells expressing FSH Receptor (Cat. No. T0605), researchers can compare the LH and FSH signaling in homologues cell systems. Phenotypic response to FSH was characterized by shape changes (cells rounding up).
