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Biochemie
Imunoanalýza
Immortalized Rat Suprachiasmatic Nucleus Cells (SCN2.2)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Brain |
| Organism |
Rat (R. norvegicus) |
| Donor History |
Long-Evans Rat ,fetal |
| Growth Properties |
Adherent ,flat rounded appearance |
| Growth Conditions |
Grow cells in poly-D-lysine or laminin-coated cell culture vessels. PriGrow VII (TM017) + 10% FBS + 2 µg/ml glucose + 2 mM L-glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂ |
| Immortalization Method |
Serial passaging and transduction with retrovirus carrying adenovirus 12S E1A gene |
| Full Information |
https://www.abmgood.com/immortalized-rat-suprachiasmatic-nucleus-cells-scn2-2.html |
Immortalized Rat Suprachiasmatic Nucleus Cells (SCN2.2) is pluripotent and has the appearance of both neuronal and glial morphologies in culture. The cells are able to differentiate into different phenotypes, where neuronal markers are expressed (NSE, PGP, and MAP-2), but glial markers are absent. It is a valuable tool for studies relating to the regulation of the central mammalian pacemaker function, and the development of the suprachiasmatic nucleus cell types. Immunofluorescent staining and western blot analysis detected for the presence of E1A.
