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Imunoanalýza
Immortalized Sweat Gland Myoepithelial Cell Line (iEM)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Sweat Gland |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,53 ,Eyelid |
| Growth Properties |
Suspension ,round spheroid |
| Growth Conditions |
Ultra-Low Attachment Plates or upright culture flasks are required for cell growth; cells must remain in suspension in order to form spheroids. Mammocult Human Medium Kit (Stemcell Technologies ,Cat. 05620) + 10 ng/ml EGF (Z100139) + 10 ng/ml bFGF (Z101456) + 4 µg/ml heparin + 0.5 µg/ml hydrocortisone 21-hemisuccinate + 2% (v/v) growth factor reduced matrigel (optional) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂.Note: thawed cells should be re-suspended in media supplemented with serum to neutralize DMSO. Cells take additional time to recover post-thaw and display cell death during the initial recovery phase. Grow cells in ultra-low attachemnt plates OR upright flask to allow spheroids to form. Subculture every 7 days and at least twice before experimental use. Seeding density: 2500 cells/well. |
| Immortalization Method |
Immortalized with lentiviral vector containing hTERT & SV40T |
| Full Information |
https://www.abmgood.com/immortalized-sweat-gland-myoepithelial-cells.html |
This novel, immortalized ecrine sweat gland myoepithelial cell line (iEM) serves as a model for research on sweat glands and sweating. These cells were developed from primary cells transduced with lentiviral vectors containing hTERT and SV40T. Cells display spheroid formation with increased population doubling compard to non-transduced cells. iEM’s express myoepithelial cell-specific proteins (a-SMA, B1 or K14) and can differentiate into luminal cells. Immortalized Sweat Gland Myoepithelial Cells provide a useful in vitro model for recapitulating eccrine sweat gland functions and for screening substances that can modulate sweating by acting on sweat glands. These cells are applicable for studies in hyperhidrosis, adiaphoresis, and sweat gland diseases. Please note: cells are exected to grow to 65 population doublings (PDs), however, the exact PD limit can vary by lot.
