KCC2 Stable HEK293 Cell Line

Potassium-chloride transporter member 5 (SLC12A5, also known as KCC2) is a neuron-specific K-Cl symporter responsible for maintaining ion homeostasis in the central nervous system. The electrochemical chloride gradient established by KCC2 activity is critical in regulating postsynaptic inhibition, as well as in protecting cells from stimulation-induced excitotoxicity. The KCC2 Stable HEK293 cell line stably over-expresses the neuronal-specific K-Cl co-transporter isoform KCC2. In contrast to KCC1, where its activity is stimulated by osmotic swelling, KCC2 activity is increased when stimulated with NEM (N-ethylmaleimide). This cell line is suitable for central nervous system studies in the potential roles of KCC2 in ion homeostatsis.The KCC2-overexpressing cell line is generated by inserting the ORF of
the rat KCC2 in the mammalian expression vector pIRES-Puro2.