Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
LUJ-35T Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Lung |
| Organism |
Human (H. sapiens) |
| Donor History |
Lung Carcinoma |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow II (TM002) + 5% FBS + 1% NEAA + 1 mM Sodium Pyruvate + 10 mM HEPES + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. Cells are sensitive to freeze/thaw cycles; supplementing media with 20% FBS is recommended during the initial thaw. Once cells recover ,5% FBS is sufficient. Cells are sensitive to trypsin; TrypLE Express is recommended for subculturing procedure. |
| Immortalization Method |
Tumor Cell Line |
| Full Information |
This tumor cell line is cultured from patient tumor tissue; cells were passaged 30 times to confirm immortalization. Cell characterization was conducted through STR of the patient tumor and cell line.
