P3X63Ag8.653 Cells

Culture medium: RPMI 1640

w: 2.0 mM stable Glutamine

w: 2.0 g/L NaHCO3 (Cytion article number 820700a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Seeding density: Start new cultures at 4 x 10⁵ cells/ml. The cell density should not exceed 2 x 10⁶ cells/ml. Freeze medium: As a cryopreservation medium

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

or CM-1 (Cytion catalog number 800100)

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C

5% CO2

humidified atmosphere.