Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
PPOX-Arg38 Stable Knockout U-2 OS Cell Line (PPOXArg38: R38/1)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Bone |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,15 ,Osteosarcoma |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/PPOX-Arg38-Stable-Knockout-U-2-OS-Cell-Line-PPOXArg38:-R38/1-T3478.html |
The knockout of Arg38 in the PPOX gene in this U-2 OS cell line, using CRISPR/Cas9 genome editing technology, leads to a complete loss of PPOX enzymatic activity, showcasing the cellular effects of inhibited heme biosynthesis. Characterized by a stark increase in protoporphyrinogen IX levels and reduced heme content, these cells maintain normal proliferation rates but exhibit compromised mitochondrial respiratory function. This cell line serves as a vital resource for probing into the cellular mechanisms underpinning heme biosynthesis disorders and evaluating potential pharmacological interventions.Complete list of cell lines from this panel:Cat. T3477 – PPOX-Cys459 Stable Knockout U-2 OS Cell Line (PPOXCys459: C459/1)Cat. T3478 – PPOX-Arg38 Stable Knockout U-2 OS Cell Line (PPOXArg38: R38/1)Cat. T3479 – PPOX-Trp227 Stable Knockout U-2 OS Cell Line (PPOXTrp227: W227/1)
