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PRC1-mCherry HeLa Stable Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Cervix |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,31 ,African American ,Cervial cancer |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
This HeLa cell line stably expresses m-Cherry tagged Protein Regulator of Cytokinesis 1 (PRC1) allowing visualization of PRC1’s localization and function during cell division. PRC1 is crucial for antiparallel microtubule bundling during mitosis, which is essential for spindle assembly and cytokinesis. This cell line enables detailed study of mitotic spindle dynamics and PRC1’s role in microtubule organization, making it an invaluable tool for research into cell division, mitotic spindle assembly, and cancer biology where mitotic errors can lead to aneuploidy and tumorigenesis.Additional cell line of interest:Cat. T3678 – mCherry-TPX2 HeLa Stable Cell Line
