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PROL1 Over-expressing Prostate Cancer Cell Line (LNCaP-PROL1+)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Prostate |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,Caucasian ,50 ,Prostate carcinoma |
| Growth Properties |
Adherent ,Epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.5 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
PROL1 overexpresison has been associated with cancer, playing a role in overcoming the hypoxic barrier that develops as tumors grow. LNCaP cells were transduced with commercially available human PROL1 lentival particles according to manufacturer protcol and slected with 5 ug/ml puroycin. RNAseq and qt-RT- PCR was used to confirm overexpression of PROL1. There was >28,560-fold overexpression of PROL1 in LNCaP-ProL1+ relative to LNCaP (by qt-RT-PCR). Cells overexpressing PROL1 show greater cell motility and migration compared to parental cells.
