Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
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Jednokanálové
Multikanálové
Sérologické pipety
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PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
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Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Prostate Cancer Cell Line (ACRJ-PC28)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Prostate |
| Organism |
Human (H. sapiens) |
| Donor History | |
| Growth Properties |
Adherent; flattened to cuboidal after passaging |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow X Series Medium for T0398 (TM0398) + 5 ng/mL EGF + 50 μg/mL bovine pituitary extract + 100 ng/mL hydrocortisone + 5 μg/mL insulin + 5 μg/mL Apo-Transferin + 0.5 ng/mL recombinant TGFα + 1 μmol/L Epinephrine + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method |
Transduction with hTERT and simultaneous suppression of CDKN2A. |
| Full Information |
https://www.abmgood.com/Prostate-Cancer-Cell-Line-ACRJ-PC28-.html |
The ACRJ-PC28 cell line is of neuroendocrine phenotype and does not express prostate-specific genes (AR, NKX3.1, and PSA) at the protein level but showed expression of NE specific markers (SYP and ENO2) at the RNA-Seq level. The cell line is atypical in expressing both basal (CK5 and p63) and luminal (CK18) markers. ACRJ-PC28 lacked expression from the CDKN2Alocus.
