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PTEN Stable Knockout MEF Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Embryo |
| Organism |
Mouse (M. musculus) |
| Donor History | |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
Gelatin Coating Solution (TM063) is required for cell adhesion to the culture vessels. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/pten-stable-knockout-mef-cell-line.html |
PTEN Stable Knockout MEF Cell Line were established via the 3T9 protocol and the expression of PTEN was knocked out. MEF cells were derived from wild-type mouse with stable expression of PTEN. PTEN (Phosphatase and tensin homolog deleted on chromosome 10) is a tumor suppressor gene and when mutated or deleted have been observed in primary human cancers. The PTEN Stable MEF Cell Line (available at abm; Cat. No. T6180) may be used alongside with the PTEN Stable Knockout MEF Cell Line to study PTEN and it’s association with cancer biology and prognosis.
