RFP Stably Expressing EOC 13.31 Cell Line

Unit	1×10⁶ cells / 1.0 ml
Cell Type	Stable (KO/Expressing) Cells
Tissue Type	Brain
Organism	Human (H. sapiens)
Donor History	Female , 10 days , microglial cell
Growth Properties	Adherent , polygonal
Growth Conditions	PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) , High Glucose (TM500) + 10% FBS + 20% LADMAC Conditioned Media (direct supernatant from the LADMAC cell line) + 1% Penicillin/Streptomycin Solution (G255) , 37.0°C , 5% CO₂. 4.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions.
Immortalization Method
Full Information	https://www.abmgood.com/rfp-stably-expressing-eoc-13-31-cell-line.html

Unit

1×10⁶ cells / 1.0 ml

Cell Type

Stable (KO/Expressing) Cells

Tissue Type

Brain

Organism

Human (H. sapiens)

Donor History

Female

10 days

microglial cell

Growth Properties

Adherent

polygonal

Growth Conditions

PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM)

High Glucose (TM500) + 10% FBS + 20% LADMAC Conditioned Media (direct supernatant from the LADMAC cell line) + 1% Penicillin/Streptomycin Solution (G255)

37.0°C

5% CO₂. 4.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions.

Immortalization Method

Full Information

https://www.abmgood.com/rfp-stably-expressing-eoc-13-31-cell-line.html

Applied Biological Materials (abm) je jedním z našich klíčových dodavatelů pro biotechnologický průmysl. Společnost byla založena v roce 2004 a již více než deset let podporuje vědecké objevy v oblasti přírodních věd a vývoje léčiv. Její sídlo se nachází ve Vancouveru v Kanadě. ABM je pro nás neocenitelným partnerem zejména v  oblasti dodávek buněčných kultur, genových a virových vektorů, produktů pro molekulární biologii a PCR, nástrojů pro technologie CRISPR a RNAi, protilátek i základních reagencií pro molekulárně genetické procesy.

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