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SPINK1 Stable AsPC-1 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Pancreas |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,62 ,Caucasian ,Ascites of a patient with cancer of the pancreas |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 2.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
The SPINK1 Stable AsPC-1 Cell Line was stably transduced with lentivirus expressing the pLenti-CMV-SPINK1-2A-GFP plasmid (Cat. No. LVP320652; Accession No. BC025790) to overexpress the human SPINK1 gene and is recommended for studies in pancreatic cancer, and the effects of SPINK1 expression.
