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Stable HA-D2LR-RLuc8 Expressing HEK-293 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History |
Embryo |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% Heat-inactivated FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.0 mg/ml Geneticin/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/stable-ha-d2lr-rluc8-expressing-hek-293-cell-line.html |
HA-D2LR-RLuc8 Expressing HEK-293 cells are derived from HEK-293 cells transiently transfected with the long form of the D2 dopamine receptor (D2L-R). D2L-R constructs were C-terminally tagged with RLuc8 enabling monitoring of total receptor expression. These cells are suitable for research concerning the localization/activity/expression of this alternatively spliced D2 dopamine receptor.
