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Stable HEK293 Expressing Human NLRP3 Cell Line (huNLRP3-YFP)
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History |
Embryo |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. DMEM/F-12 (1:1) Medium (TM510) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 300 µg/ml Geneticin/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/stable-hek293-expressing-human-nlrp3-cell-line-hunlrp3-yfp.html |
Stable HEK293 Expressing Human NLRP3 Cell Line (huNLRP3-YFP) were established through stable lipofectamine transfection of the YFP-tagged human NLRP3 gene to parental HEK293 cells. Monoclonal selection via 1 mg/ml G418 was conducted to maintain a selection pressure. The cell line established may be used to study NLRP3 inflammasome, which is highly expressed in macrophages, and its association with the inflammatory pathway.
