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Imunoanalýza
Stable Mouse Myeloid-derived Suppressor-like LAL Knock Out (HD1B) Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Peritoneum |
| Organism |
Mouse (M. musculus) |
| Donor History |
Male ,lal-/- mice |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,33.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
Peritoneal macrophages were collected from lal-/- male mice that had been crossbred with Immortomouse expressing a temperature-sensitive version of simian virus 40 large T antigen to generate the Stable Mouse Myeloid-derived Suppressor-like LAL Knock Out (HD1B) Cell Line. Myeloid-derived suppressor cells are myeloid progenitors that are blocked to further differentiate and may have a role in tumor proliferation. The cells lack the lysopmal acid lipase (LAL) expression in which the wild type is available for comparison, the Immortalized Mouse Myeloid-derived Suppressor-like (HD1A) Cells (abm, T0137). Together HD1A and HD1B cells may be used as an in vitro system for studies relating to LAL and myeloid functions.
