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Stable PML Knockout Hep-2 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Cervix |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,50 ,African American ,Human papillomavirus-related endocervical adenocarcinoma |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/stable-pml-knockout-hep-2-cell-line.html |
The Stable PML Knockout Hep-2 Cell Line was generated from HEp-2 cells by transfection of CRISPR/Cas9 targeting exon 1 of promyelocytic leukemia protein (PML). PML is a component of nuclear domain 10 (ND10) and an antiviral effector of IFN- β. In the absence of PML, the antiviral effects of IFN- β are significantly reduced. This makes the Stable PML Knockout Hep-2 Cell Line a useful model in antiviral treatments and experiments. The PML gene is also associated with a wide range of important cellular processes including tumor suppression, transcriptional regulation, apoptosis, senescence, and DNA damage response which can all be further studied using this cell line.
