Genetika člověka
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Imunoanalýza
Stable Wildtype BJAB Cell Line (BJAB-YFP)
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Blood |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,5 ,African ,Burkitt lymphoma |
| Growth Properties |
Suspension ,round |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 2.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/stable-wildtype-bjab-cell-line-bjab-yfp.html |
Wildtype BJAB stable cell line (BJAB-YFP) is made via via infection with lentiviral particles carrying pLVxAcYFP-C1-Flag. This cell line may be used alongside LANA-expressing BJAB stable cell line (BJAB-L-YFP) available from abm; Cat. No. T6314 to study LANA and its association with latency in infected cells.
