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Top3b CRISPR Knockout 17CL-1 Stable Cell Line (Top3b-KO4 17CL-1)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Embryo |
| Organism |
Mouse (M. musculus) |
| Donor History |
Sex unspecified ,14-17FD |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
The Top3b CRISPR Knockout 17CL-1 Stable Cell Line (T3605) is another variant engineered by CRISPR-Cas9 to disrupt the Topoisomerase 3b (TOP3B) gene. This cell line focuses on studying the effects of TOP3B inactivation on mRNA translation and turnover, with particular emphasis on its interaction with TDRD3 and its role in neurogenesis and synaptic plasticity. The knockout is targeted to a different region of the TOP3B gene compared to other variants, offering insights into the specific functional domains of TOP3B. It is an excellent model for exploring the implications of TOP3B loss in neurological diseases and RNA-based therapies.Complete list of cell lines from this panel: Cat. T3603 – Tdrd3 CRISPR Knockout 17CL-1 Stable Cell Line (Tdrd3-KO1 17CL-1)Cat. T3604 – Top3b CRISPR Knockout 17CL-1 Stable Cell Line (Top3b-KO2 17CL-1)Cat. T3605 – Top3b CRISPR Knockout 17CL-1 Stable Cell Line (Top3b-KO4 17CL-1)
