ts-101 Stable Knockout HEK293 Cell Line (ts-101 293KO)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History |
Embryo |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/ts-101-stable-knockout-hek293-cell-line-ts-101-293ko.html |
tRNA-derived small RNAs (tsRNAs) are dysregulated in cancer. Specfically, ts-101 is down-regulated in chronic lymphocytic leukemia and lung cancer and assocated with PiwiL2 (a protein involved in transposon silencing). The knockout cell lines model features a knockout of ts-101 in HEK293 cells using CRISPR Cas9 and sorted through limiting dilutions to obtain a single isolate cell clone.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
