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TSPO Stable Knockdown Huh7 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Liver |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,57 ,Asian ,Adult hepatocellular carcinoma |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% Heat-inactivated FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 2.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/tspo-stable-knockdown-huh7-cell-line.html |
TSPO plays a critical role in the segregation and transport of free cholesterol (FC) from the outer to the inner mitochondrial membrane in cells. Wild type Huh7 (WT) is a well-differentiated human hepatocyte-derived carcinoma cell line which contains TSPO in mitochondria. The stable TSPO knockdown (TSPO KD) in the Huh7 cell line (PBR shRNA plasmid (TSPO KD)) was made to mimic hepatic steatosis in vitro.
