Wil2-S Cells

WIL2-S is a human B-lymphoblastoid cell line derived from peripheral blood lymphocytes and immortalized by Epstein-Barr virus (EBV) infection. It originates from a healthy adult donor and exhibits characteristics typical of EBV-transformed B cells, including suspension growth, expression of B-cell surface markers, and stable proliferation in standard lymphoid culture media. As a non-tumor-derived, EBV-immortalized lymphoblastoid line, WIL2-S is widely used as a reference model in immunology, genotoxicity, and DNA repair studies.

WIL2-S has been extensively applied in cytogenetic and mutagenesis assays, particularly in micronucleus and chromosomal instability testing, due to its stable karyotype and well-characterized response to DNA-damaging agents. The cell line is proficient in DNA repair pathways and has served as a comparator in studies evaluating oxidative stress, radiation-induced damage, and chemotherapeutic genotoxicity. Its lymphoid origin and reproducible growth characteristics make it suitable for evaluating clastogenic and aneugenic effects in vitro under controlled experimental conditions.

Because WIL2-S is not derived from a malignant tumor but rather represents an EBV-immortalized normal B-cell lineage, it provides an important baseline model for distinguishing cancer-specific molecular alterations from general lymphoid cellular responses. Researchers commonly use this cell line as a non-transformed reference in studies of genome stability, immune cell signaling, and mechanisms of cellular stress response.