c-Kit/WT Stable Ba/F3 (with GNNK- isoform) Cell Line

The GNNK- isoform is the outcome of the c-Kit gene alternative splicing sites, resulting in different isoforms of the tetrapeptide GNNK, and is the main transcript found in human malignancies. Signaling molecules Cbl, Shc, Gab2, Erk and Akt are more strongly activated by the presence of the GNNK- isoform. These cells will proliferate in the presence of the ligand SCF and activate downstream signal transduction. Rapid degradation of the SCF is observed in cells expressing the GNNK-, as the Src inhibitor SU6656 (SUGEN) exerts stronger effects when compared with GNNK isoform cells.Apart from the GNNK- isoform, GNNK isoform c-Kit wild-type Stable Ba/F3 cells are also available (Cat. No. T3042). Together with c-Kit mutated (i.e. D816V, Y568F, Y570F, Y703F) GNNK- isoform Ba/F3 cells, these cell lines are suitable for studies in drug discovery for mutations in the c-Kit receptor which are associated to oncogenic and other diseases.