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Akt1 Stable Expressing NIH3T3 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Embryo |
| Organism |
Mouse (M. musculus) |
| Donor History |
Male ,NIH Swiss ,Embryo |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 3.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/akt1-stable-expressing-nih3t3-cell-line.html |
NIH3T3 cells were transfected with the pLKO-AS3W-Puro-flag vector carrying the Akt1 wildtype gene. Puromycin selection was completed to establish the Akt1 Stable Expressing NIH3T3 Cell Line. The cells are a great tool to study Akt1 which is a serine/threonine kinase involved in processes such as cell proliferation/survival/metabolism and if hyperactivated is linked to cancer progression.
