Ana-1 Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Mouse cell lines |
| Organism |
Mouse |
| Donor History | |
| Growth Properties |
Adherent/Suspension |
| Growth Conditions |
Culture medium: RPMI 1640 ,w: 2.0 mM stable Glutamine ,w: 2.0 g/L NaHCO3 (Cytion article number 820700a). Supplements: Supplement the medium with 10% heat-inactivated FBS. Dissociation reagent: Accutase. Seeding density: 2 to 4 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
The Ana-1 cell line is a murine macrophage cell line derived from the ascites of a BALB/c mouse with a methylcholanthrene-induced tumor. This cell line is extensively used in immunological research due to its ability to produce a variety of cytokines and its role in the innate immune response. Ana-1 cells exhibit phagocytic activity and are capable of producing nitric oxide (NO), which is crucial for the cytotoxic response against pathogens and tumor cells.
Furthermore, Ana-1 cells have been employed as a model system to study macrophage activation and the signaling pathways involved in immune responses. The cells respond to lipopolysaccharide (LPS) stimulation by upregulating the expression of pro-inflammatory cytokines, making them suitable for investigations into the molecular mechanisms of inflammation and the host defense system. Their compatibility with various immunological assays also makes them valuable for drug screening and for understanding macrophage interactions with other cell types in the immune system.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza

