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AP‐1‐luc‐CMVH Stably Expressing RAW264.7 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Ascites |
| Organism |
Mouse (M. musculus) |
| Donor History |
Male ,Balb/c ,Adult ,Abelson murine leukemia virus-induced tumor |
| Growth Properties |
Adherent ,dendritic-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. MEM α ,No Nucleosides Medium (TM512) + 10% FBS + 2 mM L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
The RAW-AP-1-luc-CMVH Stable RAW264.7 Cell Line is an invaluable tool for investigating the activator protein-1 (AP-1) pathway, critical for immune responses and cellular growth processes. Engineered from the RAW264.7 murine macrophage cell line, it includes a luciferase reporter gene driven by an AP-1-responsive promoter enhanced by a cytomegalovirus (CMV) promoter. This configuration allows for the real-time, quantitative analysis of AP-1 activity following stimulation by factors like cytokines and growth factors, making it ideal for studies on inflammation, oncogenesis, and the screening of potential therapeutic agents targeting AP-1 mediated pathways.Complete list of cell lines from this panel:Cat. T6529 – NFAT‐luc‐CMVH Stably Expressing RAW264.7 Cell LineCat. T6530 – AP‐1‐luc‐CMVH Stably Expressing RAW264.7 Cell LineCat. T6531 – ARE‐luc‐CMVH Stably Expressing RAW264.7 Cell Line
