AT1A/AT1B Double Knockout Immortalized Mouse Renal Proximal Tubule Cell Line

The proximal tubule is part of the nephron duct system in the kidney, involved in regulating the renin-angiotension system. Cells of this region aid in regulating sodium and volume homeostasis as well as regulating the systemic blood pressure. The protein Angiotension II (Ang II) and its receptors AT1 and AT2 are some of the most studied proteins in this system. Even so, there is an incomplete understanding of the AT1 receptors AT1A and AT1B. The AT1B Knockout Immortalized Mouse Renal Proximal Tubule Cell Line is a conditionally immortalized mouse renal proximal tubule cells isolated from the mouse harboring thermolabile mutation (tsA58) of the simian virus 40 large T antigen. The functional expression of the SV40 large T antigen is induced by culturing the cells in vitro in medium containing IFN γ at a temperature permissive (33°C). At a non-permissive temperature (37°C-39°C), the cells cease to proliferate. When paired up with the wild type (Cat. No. T0624) and other angiotensin receptor (Ang II)-deficient cell lines AT1A-/- (Cat. No. T0626), AT1B-/- (Cat. No. T0627), and AT1A-/- AT1B-/- (Cat. No. T0628), these cell lines represent valuable tools to study fluid and electrolyte transportation in the proximal tubule region. RT-PCR was performed to confirm AT receptor genotype.