ATG4B Stable Knockout HeLa Cell Line

Unit

1×10⁶ cells / 1.0 ml

Cell Type

Stable (KO/Expressing) Cells

Tissue Type

Cervix

Organism

Human (H. sapiens)

Donor History

Growth Properties

Adherent

epithelial-like

Growth Conditions

PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM)

High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1 mM Sodium Pyruvate (TM057) + 1% Penicillin/Streptomycin Solution (G255)

37.0°C

5% CO₂.

Immortalization Method

Full Information

The ATG4B Stable Knockout HeLa Cell Line was developed using CRISPR-Cas9 technology to specifically disrupt the ATG4B gene, a key cysteine protease involved in autophagy, particularly in the lipidation and processing of LC3 and GABA type A receptor-associated protein (GABARAP) family proteins. This cell line exhibits a severe but incomplete defect in autophagy, characterized by an impaired ability to lipidate LC3B, resulting in a significant reduction in autophagosome formation and cargo degradation. However, residual lipidation of GABARAPL1 and GABARAPL2 can still occur due to the compensatory activity of other ATG4 isoforms. This unique cell line is invaluable for research focused on dissecting the specific roles of ATG4B in autophagy and its contribution to cellular processes such as protein aggregation, intracellular trafficking, and degradation.Complete list of cell lines from this panel:Cat. T3588 – ATG4B Stable Knockout HeLa Cell LineCat. T3589 – ATG4A/B Stable Double Knockout HeLa Cell LineCat. T3590 – ATG4A/B/C Stable Triple Knockout HeLa Cell Line

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Unit	1×10⁶ cells / 1.0 ml
Cell Type	Stable (KO/Expressing) Cells
Tissue Type	Cervix
Organism	Human (H. sapiens)
Donor History
Growth Properties	Adherent , epithelial-like
Growth Conditions	PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) , High Glucose (TM500) + 10% FBS + 2 mM L-Glutamine (G275) + 1 mM Sodium Pyruvate (TM057) + 1% Penicillin/Streptomycin Solution (G255) , 37.0°C , 5% CO₂.
Immortalization Method
Full Information

Přidat mezi Oblíbené

Katalogové číslo: T3588 Kategorie: Buněčné linie, Stable (KO/Expression) Cell Lines

Popis
O značce

Popis

O značce

Applied Biological Materials (abm) je jedním z našich klíčových dodavatelů pro biotechnologický průmysl. Společnost byla založena v roce 2004 a již více než deset let podporuje vědecké objevy v oblasti přírodních věd a vývoje léčiv. Její sídlo se nachází ve Vancouveru v Kanadě. ABM je pro nás neocenitelným partnerem zejména v  oblasti dodávek buněčných kultur, genových a virových vektorů, produktů pro molekulární biologii a PCR, nástrojů pro technologie CRISPR a RNAi, protilátek i základních reagencií pro molekulárně genetické procesy.

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