Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
Zkumavky
Třepačky
Suché lázně
Centrifugy
End Point cyclery
Real-time PCR cyclery
Fluorometry
Spektrofotometry
Izolátory nukleových kyselin
Pipetovací roboty
Biobanking
Live Cell Imaging
Jednokanálové
Multikanálové
Sérologické pipety
Jednorázové pipety
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
Bax/Bak Double Knockout Immortalized Mouse Kidney Epithelial Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Mouse (M. musculus) |
| Donor History |
C57B/6J mice |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/bax-bak-double-knockout-immortalized-mouse-kidney-epithelial-cell-line.html |
Key regulators of apoptosis, such as members of the BCL-2 family, have long been linked to the development of cancer. The Bax/Bak Double Knockout Immortalized Mouse Kidney Epithelial Cells (bax-/-/ bak-/- iBMK) is derived from stable transformation of E1A and p53DD into primary baby kidney epithelial cells from C57B/6J mice deficient for Bak and Bax. Bax and Bak are functionally redundant downstream regulators to the mechanism of apoptosis, therefore this double knockdown cell line provides a valuable insight into pro-apoptotic proteins and programmed cell death. Together with the wild-type iBMK (T0082), Bax knockout iBMK (bax-/- iBMK, T3024) and Bak Knockout iBMK (Bak-/- iBMK, T3025), these cell lines are suitable in studying the relationships between BCL-2 family and cancer progression, prognosis and significance to treatment.
