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Imunoanalýza
Bax Knockout Immortalized Mouse Kidney Epithelial Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Mouse (M. musculus) |
| Donor History |
C57B/6J mice |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/bax-knockout-immortalized-mouse-kidney-epithelial-cell-line.html |
Key regulators of apoptosis, such as members of the BCL-2 family, have long been linked to the development of cancer. The Bax Knockout Immortalized Mouse Kidney Epithelial Cells (bax-/- iBMK) is derived from stable transformation of E1A and p53DD into primary baby kidney epithelial cells from C57B/6J mice deficient for Bax. Together with the wild-type iBMK (T0082), Bak knockout iBMK (bak-/- iBMK, T3025) and Bak and Bax Double Knockout iBMK (Bak-/-/Bax-/-iBMK, T3026), these cell lines are suitable in studying the relationships between BCL-2 family and cancer progression, prognosis and significance to treatment.
