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Imunoanalýza
BRCA1 L1780P Mutant Stable HCC1937 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Mammary |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,Caucasian ,23 ,Primary ductal carcinoma TNM Stage IIB ,Grade 3 |
| Growth Properties | |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/brca1-l1780p-mutant-stable-hcc1937-cell-line.html |
This cell line stably expresses the breast cancer associated gene 1 (BRCA1) L1780P BRCT domain using the triple-negative cell line, HCC1937. Exogenous BRCA1 was confirmed by Western blot and Sanger sequencing confirmed the successful introduction of mutuant-BRCA1 in the stable cell line. These cells show increased migration and invasion capacity, as well as increased sensitivity to olaparib and carboplatin, compared to wild-type BRCA1 cells. The cell lines are suitable for breast cancer research, understanding the role of BRCA1 and for the development of TNBC treatment.
