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Cathepsin D Stable Knockout SH-SY5Y Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Nerve |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,4 ,Neuroblastoma |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. 1:1 mixture of Prigrow VIII Medium (TM018) and PriGrow XVII Medium (TM029) + 10% FBS + 1% L-Glutamine (G275) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂.Note: Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures. |
| Immortalization Method | |
| Full Information |
This cell line was generated using the Tet-On system for inducible knockdown. SH-SY5Y cells were transfected with plasmids carrying shRNA sequences targeting different regions of the Cathepsin D mRNA. Effective knockdown clones were selected using zeocin, and expression was controlled via tetracycline induction. In the absence of Cathepsin D, these cells show enhanced growth rates, especially under stimulation by EGF, indicating that Cathepsin D normally acts to suppress this growth-promoting pathway. This cell line, in conjunction with Cat. T3258, is useful for investigating the role of Cathepsin D in cancer cell growth and chemoresistance, particularly in the context of EGFR overexpression. This model is also valuable for evaluating potential cancer therapies that target or bypass Cathepsin D pathways.Additional cell lines from this panel: T3528 – Cathepsin D Stably Expressing SH-SY5Y Cell Line
