GFP Stable Expressing Bone Marrow Mesenchymal Stem Cells – Polyclonal

Unit	1×10⁶ cells / 1.0 ml
Cell Type	Stable (KO/Expressing) Cells
Tissue Type	Bone Marrow
Organism	Human (H. sapiens)
Donor History	Not disclosed
Growth Properties	Adherent , bipolar
Growth Conditions	PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. Dulbecco's Modified Eagle Medium (DMEM) , High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) , 37.0°C , 5% CO₂. 2.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions.
Immortalization Method	Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen
Full Information	https://www.abmgood.com/gfp-stably-expressing-human-bone-marrow-mesenchymal-stem-cells-sv40.html

Unit

1×10⁶ cells / 1.0 ml

Cell Type

Stable (KO/Expressing) Cells

Tissue Type

Bone Marrow

Organism

Human (H. sapiens)

Donor History

Not disclosed

Growth Properties

Adherent

bipolar

Growth Conditions

PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. Dulbecco's Modified Eagle Medium (DMEM)

High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255)

37.0°C

5% CO₂. 2.0 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions.

Immortalization Method

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

Full Information

https://www.abmgood.com/gfp-stably-expressing-human-bone-marrow-mesenchymal-stem-cells-sv40.html

Applied Biological Materials (abm) je jedním z našich klíčových dodavatelů pro biotechnologický průmysl. Společnost byla založena v roce 2004 a již více než deset let podporuje vědecké objevy v oblasti přírodních věd a vývoje léčiv. Její sídlo se nachází ve Vancouveru v Kanadě. ABM je pro nás neocenitelným partnerem zejména v  oblasti dodávek buněčných kultur, genových a virových vektorů, produktů pro molekulární biologii a PCR, nástrojů pro technologie CRISPR a RNAi, protilátek i základních reagencií pro molekulárně genetické procesy.

GFP Stable Expressing Bone Marrow Mesenchymal Stem Cells – Polyclonal

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GFP Stable Expressing Bone Marrow Mesenchymal Stem Cells – Polyclonal

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