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Imunoanalýza
HEK293 Stably Expressing cynomolgous Ephrin Type-B Receptor 4 (293-cynomolgus EPHB4.puroR.GFP)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History |
Embryo |
| Growth Properties |
Adherent |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
Ephrin type-B receptor 4 (EPHB4) is expressing in tumors and a suitable target for chimeric antigen recept (CAR)-T cells. To produce human or murine EPHB4-expressing HEK293 cells, pIRII-humanEPHB4-GFP or a pIRII-murineEPHB4-GFP plasmid, which contains GFP and human EPHB4 or murine Ephb4 were transduced into HEK293 cells. GFP expressing cells were purified via flow cytometry and the respective cell lines were established.
