HIF HRE-Luc/LacZ Stable Expressing Me1290 Human Uveal Melanoma Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Eye |
| Organism |
Human (H. sapiens) |
| Donor History |
Uveal Melanoma |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS + 1X Non-Essential Amino Acids (TM068) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 50 µg/ml Hygromycin (G265) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
HIF HRE-Luc/LacZ Stable Expressing Me1290 Human Uveal Melanoma Cell Line expresses hypoxia-responsive dual LacZ/Luciferase reporter to monitor HIF-1 activity. This cell line is useful to study the effects of HIF-1 activity.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
