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JWA Stable Knockout Human MHCC97L Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Liver |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,39 ,Hepatocellular carcinoma |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 1.5 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/jwa-stable-knockout-human-mhcc97l-cell-line.html |
JWA Stable Knockout Human MHCC97L Cell Line is established via CRISPR/Cas9 mediated frameshift mutation of the JWA gene (NM_006407) using the following guide RNA sequence: AGAGCAGGTTGCTCACTACG. JWA gene is associated with cytoskeleton and has been reported to regulate intracellular concentrations of taurine and glutamate. As such, this cell line may be useful to study membrane trafficking. This cell line is sequenced verified to be biallelic knockout.
