KC Cells

Unit

3 x 10^6 cells/ml

Cell Type

Immortalized Cells

Tissue Type

Animal cells

Organism

Drosophila melanogaster (Fruit fly)

Donor History

Normal

Growth Properties

semi-adherent

Growth Conditions

Culture medium: Schneider's Drosophila medium + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Supplements: Supplement the medium with 2 mM Glutamine and 10% FBS. Dissociation reagent: Not required (semi-adherent; resuspend gently). Seeding density: 5 × 10⁵ to 1 × 10⁶ cells/ml. Freeze medium: As a cryopreservation medium

,

we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability

,

or CM-1 (Cytion catalog number 800100)

,

which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 25°C

,

ambient air (no CO₂ required).

Immortalization Method
Full Information

https://www.cytion.com/KC-Cells/300604

SKU: 300604 Kategorie: ,
Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

KC (Kc167) is a Drosophila melanogaster embryonic cell line derived from a dorsal closure stage female embryo. The line was originally established and has been widely used in Drosophila cell biology, genetic screens, and functional genomics. Kc167 cells have been immortalized with SV40 and grow semi-adherently, forming a mixed population of attached and loosely floating cells. They are among the best-characterized Drosophila cell lines and are fully amenable to RNAi-mediated gene silencing, making them a cornerstone model for genome-wide RNAi screens in invertebrate biology.
KC cells are applicable in Drosophila cell biology, genome-wide RNAi and CRISPR screens, signal transduction pathway studies (Wnt/Wingless, Hedgehog, Notch, JAK/STAT, Toll/NF-κB), innate immunity research, and comparative studies of evolutionarily conserved pathways. The fully sequenced and annotated Drosophila genome, combined with extensive public databases of RNAi reagents (e.g., DRSC/TRiP), makes KC cells particularly powerful for unbiased functional genomic discovery. BSL-2 classification applies due to the SV40 immortalization.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.