KC Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Immortalized Cells |
| Tissue Type |
Animal cells |
| Organism |
Drosophila melanogaster (Fruit fly) |
| Donor History |
Normal |
| Growth Properties |
semi-adherent |
| Growth Conditions |
Culture medium: Schneider's Drosophila medium + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Supplements: Supplement the medium with 2 mM Glutamine and 10% FBS. Dissociation reagent: Not required (semi-adherent; resuspend gently). Seeding density: 5 × 10⁵ to 1 × 10⁶ cells/ml. Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 25°C ,ambient air (no CO₂ required). |
| Immortalization Method | |
| Full Information |
KC (Kc167) is a Drosophila melanogaster embryonic cell line derived from a dorsal closure stage female embryo. The line was originally established and has been widely used in Drosophila cell biology, genetic screens, and functional genomics. Kc167 cells have been immortalized with SV40 and grow semi-adherently, forming a mixed population of attached and loosely floating cells. They are among the best-characterized Drosophila cell lines and are fully amenable to RNAi-mediated gene silencing, making them a cornerstone model for genome-wide RNAi screens in invertebrate biology.
KC cells are applicable in Drosophila cell biology, genome-wide RNAi and CRISPR screens, signal transduction pathway studies (Wnt/Wingless, Hedgehog, Notch, JAK/STAT, Toll/NF-κB), innate immunity research, and comparative studies of evolutionarily conserved pathways. The fully sequenced and annotated Drosophila genome, combined with extensive public databases of RNAi reagents (e.g., DRSC/TRiP), makes KC cells particularly powerful for unbiased functional genomic discovery. BSL-2 classification applies due to the SV40 immortalization.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Třepačky
Suché lázně
Fluorometry
Buněčné kultury
PCR, qPCR, RT
Detekce Mycoplasmat
Reverzní transkripce
Alergie
Intolerance
Elisa
Imunologické analyzátory
Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza

