KtyI Stable Knockout Mouse Keratinocyte Cell Line

Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. DMEM/Ham’s F12

low Calcium (50µM) (Biochrom) + 10% chelexed fetal calf serum (PAA

A15-151) + 2 mM Glutamax (Invitrogen

35050-038) + 1X Pyruvate (PAA

S11-003) + 0.18mM Adenin (Sigma

A8626-5G) + 10 ng/ml EGF (Invitrogen 53003-018) + 2.5 µg/ml insulin (Sigma-Aldrich

I-9278) + 0.5 µl Hydroxycortison (Sigma-Aldrich

H4001-1G) + 10-⁻¹⁰ M Cholera toxin (Sigma

C-8052) + 1% Penicillin/Streptomycin Solution (G255)

37.0°C

5% CO₂. Selection with 0.1 µg/ml Puromycin (G264)