Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
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Pipetovací roboty
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Jednokanálové
Multikanálové
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Reverzní transkripce
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Veterinární imunologie
Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
MCFD2 Stable Knockout HepG2 Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Liver |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,15 ,White ,Hepatocellular carcinoma |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Eagle's Minimal Essential Medium (EMEM) (TM511) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/MCFD2-Stable-Knockout-HepG2-Cell-Line-T3420.html |
This HepG2 cell line features a stable knockout of MCFD2 and is applicable for studying the ER-to-Golgi transport mechanism of blood coagulation factors and proteins such as α1-antitrypsin. MCFD2, when complexed with LMAN1, is essential for the proper functioning of the coagulation factors V and VIII, which are critical for blood clotting processes. Furthermore, this cell line is suitable for dissecting the molecular roles of the MCFD2 protein in liver cells. Researchers can utilize this knockout model to explore MCFD2’s individual contributions to the secretory pathway, its interaction with LMAN1, and its impact on cellular health and disease states, particularly related to liver function and blood coagulation disorders.
