Genetika člověka
Mikrobiologické aplikace
NGS
Rapid testy
PCR plasty
Pipetovací špičky
Plasty pro buněčnou kultivaci
Systém pro dlouhodobé skladování vzorků
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Pipetovací roboty
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PCR, qPCR, RT
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Čtečky identifikačních čipů
Termočipy
Biochemie
Imunoanalýza
MIA PaCa-2 Cells
| Unit |
3 x 10^6 cells/ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Pancreas cancer cell lines |
| Organism |
Human |
| Donor History |
Ductal adenocarcinoma |
| Growth Properties |
Adherent with loosely attached rounded cells |
| Growth Conditions |
Culture medium: DMEM ,w: 4.5 g/L Glucose ,w: 4 mM L-Glutamine ,w: 3.7 g/L NaHCO3 ,w: 1.0 mM Sodium pyruvate (Cytion article number 820300a). Supplements: Supplement the medium with 10% FBS. Dissociation reagent: Accutase. Seeding density: 1 x 10⁴ cells/cm². Freeze medium: As a cryopreservation medium ,we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability ,or CM-1 (Cytion catalog number 800100) ,which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Incubation atmosphere: 37°C ,5% CO2 ,humidified atmosphere. |
| Immortalization Method | |
| Full Information |
The MIA PaCa-2 cell line is an indispensable asset in the field of cancer research and was derived from the pancreatic carcinoma tissue of a 65-year-old male. Mia PaCa 2 cells are widely used in the study of pancreatic ductal adenocarcinoma (PDAC), a notoriously aggressive and lethal cancer type. The cell line offer a solid tumor model that reflects the cellular characteristics of PDAC. One of the key attributes of this cell line is its genetic profile, which includes mutations in critical genes like KRAS and TP53, which are emblematic of the genetic landscape observed in pancreatic cancer patients.
The cells have been extensively utilized to investigate various aspects of pancreatic cancer growth, metastasis, and resistance to therapeutics. Mia Paca-2 cells are instrumental in assessing the efficacy of chemotherapeutic drugs. Furthermore, the cell line serves as a vital resource for probing into the signaling pathways pivotal for cancer cell survival and metastasis, including the MAPK, PI3K/AKT, and Wnt pathways. Studies utilizing MIA PaCa-2 cells have also shed light on the dynamic interactions between cancer cells and their microenvironment. MIA PaCa-2’s robust in vitro growth and its capacity to form tumors in xenograft models make it particularly suited for examining cancer progression and the mechanisms of tumorigenesis.
In summary, the Mia Paca-2 cell line, with its broad application in pancreatic cancer research, continues to be a critical resource for scientists worldwide.
Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.
Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.
