Genetika člověka
Mikrobiologické aplikace
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Jednokanálové
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PCR, qPCR, RT
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Reverzní transkripce
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Imunologické analyzátory
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Alergie u zvířat
Intolerance u zvířat
Biochemie
Imunoanalýza
NIH/3T3 Stably Expressing L-SIGN Receptor Cell Line (NIH3T3/L-SIGN)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Embryo |
| Organism |
Mouse (M. musculus) |
| Donor History |
Male ,NIH Swiss ,Embryo |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/NIH-3T3-Stably-Expressing-L-SIGN-Receptor-Cell-Line-NIH3T3-L-SIGN-t3391.html |
L-SIGN (liver/lymph node SIGN/ CD209L) is expressed on the surface of dendritic cells and promotes HIV and SIV infection of CD4+ T cells. NIH/3T3 cells were stably transduced with vectors encoding DC-SIGN and sorted via fluorescence-activated cell sorting (FACS) with the L-SIGN-cross-reactive MAb 526(X). These cell lines (Cat. T3390 and T3391) are suitable for studies investigating the role of DC-SIGN and L-SIGN mediated vrius transmission.
