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R49A αA-crystallin Stable Expressing CHO Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Ovary |
| Organism |
Chinese Hamster (C. griseus) |
| Donor History |
Female ,Adult ,Chinese hamster |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Ham's F-12 Medium + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 400 µg/ml Geneticin/G418 (G271) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/r49a-aa-crystallin-stable-expressing-cho-cell-line.html |
R49A αA-crystallin Stable Expressing CHO Cell Line was designed to express the mutant R49A αA-crystallin to study the effects the mutation has for protein function as a molecular chaperone, an antiapoptotic protein, and as a structural protein of the lens. αA-crystallin Stable Expressing CHO Cell Line (T6236) and R12A αA-crystallin Stable Expressing CHO Cell Line (T6234) are also available from abm.
