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Stable Human Hepatic Stellate Expressing DDX5-WT Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Liver |
| Organism |
Human (H. sapiens) |
| Donor History | |
| Growth Properties |
Adherent ,polygonal |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 500 µg/ml Zeocin for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/stable-human-hepatic-stellate-expressing-ddx5-wt-cell-line.html |
Stable Human Hepatic Stellate Expressing DDX5-WT Cell Line is derived from the spontaneously-immortalized human stellate cell line (LX-2) in which was infected with the non-replicating lentivirus carrying the FLAG-tagged DDX5 WT vector.
