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Top3b CRISPR Knockout 17CL-1 Stable Cell Line (Top3b-KO2 17CL-1)
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Embryo |
| Organism |
Mouse (M. musculus) |
| Donor History |
Sex unspecified ,14-17FD |
| Growth Properties |
Adherent ,fibroblast |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
The Top3b CRISPR Knockout 17CL-1 Stable Cell Line (T3604) was engineered to knock out the Topoisomerase 3b (TOP3B) gene using CRISPR-Cas9 technology. TOP3B is a unique enzyme with dual DNA and RNA topoisomerase activities, playing crucial roles in transcriptional regulation, RNA metabolism, and maintaining genomic stability. This knockout cell line provides a powerful tool for studying the impact of TOP3B loss on cellular processes, including replication stress, mRNA translation, and the development of neurodevelopmental disorders. It is especially useful in research related to RNA topological problems and potential therapeutic targeting of TOP3B in diseases.Complete list of cell lines from this panel: Cat. T3603 – Tdrd3 CRISPR Knockout 17CL-1 Stable Cell Line (Tdrd3-KO1 17CL-1)Cat. T3604 – Top3b CRISPR Knockout 17CL-1 Stable Cell Line (Top3b-KO2 17CL-1)Cat. T3605 – Top3b CRISPR Knockout 17CL-1 Stable Cell Line (Top3b-KO4 17CL-1)
