UM-SCC-112 Cells

Unit

3 x 10^6 cells/ml

Cell Type

Tumor Cells

Tissue Type

Head-Neck cancer cell lines

Organism

Human

Donor History

Head and neck squamous cell carcinoma (HNSCC)

Growth Properties

Adherent

Growth Conditions

Culture medium: DMEM

,

w: 4.5 g/L Glucose

,

w: 4 mM L-Glutamine

,

w: 3.7 g/L NaHCO3

,

w: 1.0 mM Sodium pyruvate (Cytion article number 820300a). Supplements: Supplement the medium with 10% FBS and 1% non-essential amino acids (NEAA). Dissociation reagent: Accutase. Seeding density: 1 to 3 × 10⁴ cells/cm². Freeze medium: As a cryopreservation medium

,

we use complete growth medium + 10% DMSO for adequate post-thaw viability. Incubation atmosphere: 37°C

,

5% CO2

,

humidified atmosphere.

Immortalization Method
Full Information

https://www.cytion.com/UM-SCC-112-Cells/305720

SKU: 305720 Kategorie: ,
Mgr. Otakar Fišer
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Mgr. Otakar Fišer
Produktový manažer - Life Science
Popis

UM-SCC-112 is a human head and neck squamous cell carcinoma (HNSCC) cell line established at the University of Michigan Head and Neck Oncology laboratory as part of the UM-SCC panel. The UM-SCC series represents one of the most extensively characterized and widely distributed HNSCC cell line collections in the world, derived from patients with oral cavity, laryngeal, hypopharyngeal, and oropharyngeal squamous cell carcinomas treated at the University of Michigan Medical Center. UM-SCC-112 grows as an adherent monolayer with epithelial-like morphology and is used as part of a multi-line panel for comprehensive preclinical characterization of HNSCC biology.
UM-SCC-112 is applicable in studies of HNSCC biology, drug sensitivity and resistance, radiation response, EGFR and other signalling pathway analyses, and comparative molecular characterization within the UM-SCC panel. The line is suitable for pharmacological assays, invasion and migration studies, and xenograft models in immunocompromised hosts. Site-specific and donor-specific details for UM-SCC-112 should be verified from the University of Michigan SPORE Tissue Core for the most current annotations.

O značce

Společnost Cytion vznikla v roce 2004 v německém Heidelbergu s jasným vědeckým posláním: dodávat ověřené lidské a zvířecí buněčné linie bez kontaminace, připravené za přísně kontrolovaných laboratorních podmínek.

Postupně se vypracovala ve spolehlivou buněčnou banku, která slouží akademickým laboratořím, biotechnologickým firmám i farmaceutickým výzkumným týmům po celém světě. Klade přitom důraz na reprodukovatelnost výsledků, přísnou kontrolu kvality a dlouhodobá partnerství.