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Biochemie
Imunoanalýza
YMAC1 Cells
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Tumor Cells |
| Tissue Type |
Liver |
| Organism |
Mouse (M. musculus) |
| Donor History |
Double knockout Glycine N-Methyltransferase( GNMT) mice ,22 months ,hepatocellular carcinoma |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS + 2 mM L-glutamine + 1X NEAA (TM068) + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂ |
| Immortalization Method |
Tumor Cell Line |
| Full Information |
YMAC1 are tumor derived cells that were isolated from the livers of double knockout Glycine N-Methyltransferase( GNMT) mice; a genotype which subsequently resulted in the development of hepatocellular carcinmoma (HCC). Along with the expression of high levels of Survivin and Glypican 3, allografts of YMAC1 cells have also shown sarcomatoid HCC characteristics, such as high levels of expression of cytokeratin 8 (CK8) and cytokeratin 7 (CK7). These tumorgenic cells also express epithelial to mesenchymal transition characteristics with robust of expression of both N-cardherin and vimentin but low expression of E-cadherin and fast motility during wound healing.
