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Imunoanalýza
AMY3 Stable HEK293 Cell Line
| Unit |
1×10⁶ cells / 1 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Kidney |
| Organism |
Human (H. sapiens) |
| Donor History |
Embryo |
| Growth Properties |
Adherent ,epithelial-like |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 100 ng/ml Zeocin for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
Amylin receptor-3 (AMY3) is an amylin receptor that is suspected to play a role in both Abeta and hAmylin downstream signaling. Amylin receptors belong to class B GPCRs and are heterodimeric complexes formed by calcitonin receptor (CTR) association with receptor activity modifying proteins (RAMPs). Activation of amylin receptors in mammalian cells has been shown to raise cAMP and presumed to involve GS protein (Gs -guanine nucleotide-binding signal transduction protein) and stimulation of adenylate cyclase. AMY3 Stable HEK293 Cell Line allows for the study of the intracellular signal transduction pathways that are activated by Abeta or hAmylin and to further investigate whether their cytotoxic effects are mediated via this particular isoform of the amylin receptor.
