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C9orf72 CRISPR Knockout HeLa Cell Line
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Cervix |
| Organism |
Human (H. sapiens) |
| Donor History |
Female ,31 ,African American ,Cervial cancer |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. |
| Immortalization Method | |
| Full Information |
This HeLa cell line features a CRISPR/Cas9 mediated stable knockout of C9orf72 which binds to SMCR8, localizes to lysosomes, and plays a critical role in regulating mTORC1 signaling. The knockout of C9orf72 revealed phenotypes such as abnormally swollen lysosomes and altered mTORC1 signaling responses, highlighting its importance in lysosomal function and cellular homeostasis. Complete list of cell lines from this panel: Cat. T3807 – C9orf72 CRISPR Knockout HeLa Cell LineCat. T3808 – SMCR8 CRISPR Knockout HeLa Cell LineCat. T3809 – C9orf72-SMCR8 CRISPR Double Knockout HeLa Cell Line
