Genetika člověka
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Imunoanalýza
GFP Control A2058 Cells (Lenti-CMV-CBH-GFP-2A-Puro-Blank )
| Unit |
1×10⁶ cells / 1.0 ml |
|---|---|
| Cell Type |
Stable (KO/Expressing) Cells |
| Tissue Type |
Skin |
| Organism |
Human (H. sapiens) |
| Donor History |
Male ,43 ,White ,Melanoma |
| Growth Properties |
Adherent ,epithelial |
| Growth Conditions |
PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Dulbecco's Modified Eagle Medium (DMEM) ,High Glucose (TM500) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255) ,37.0°C ,5% CO₂. 0.2 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. |
| Immortalization Method | |
| Full Information |
https://www.abmgood.com/GFP-Control-A2058-Cells-Lenti-CMV-CBH-GFP-2A-Puro-Blank-T9812.html |
This GFP Control A2058 cell line stably expresses Lenti-CMV-CBH-GFP-2A-Puro-Blank. These cells express Green Fluorescent Protein (GFP), enabling easy visualization and tracking in a variety of experimental conditions. Originating from the A2058 cell line, popular in melanoma studies, these cells are modified to express GFP via a lentiviral system under the control of the CMV (Cytomegalovirus) promoter. This feature, combined with the 2A-Puro-Blank selection marker, allows for the selection of successfully transduced cells using puromycin, making it an invaluable tool for researchers studying cancer biology, gene expression, and protein localization. The versatility and robust expression system make these cells ideal for a broad range of applications, including but not limited to, gene function studies, drug screening, and cell signaling pathways investigation.
